ysxC [2019-09-20 09:49:06]

assembly of the 50S subunit of the ribosome

Locus: BSU_28190

Isoelectric point: 9.71

Molecular weight: 21.88 kDa

Protein length: 195 aa

Gene length: 588 bp

Function: ribosome assembly

Product: GTPase

Essential: yes

Synonyms: orfX

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
• Groups of genes → Essential genes
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 2,879,882 → 2,880,469

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes GTP and readily exchanges GDP for GTP
• participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit PubMed

Protein family

• TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)

Paralogous protein(s)

• RbgA, ThdF, Obg, YqeH, YnbA, YphC, YyaF, Era

Structure

• 1SVI (complex with GDP) PubMed
• 1SUL PubMed

Localization

• cytosol (according to UniProt)

Expression and Regulation

Operons

• Genes: lonA-ysxC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon

  Regulation

  • induced by heat stress (CtsR) PubMed

Biological materials

Mutant

• MGNA-A996 (ysxC::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

Loading

Original publications

Loading